Il Miami Cancer Institute ha condotto la ricerca virologica utilizzando il kit di estrazione RNAdvance Viral
Customer Spotlight: Beckman Coulter Life Sciences had the honor of interviewing Weiming Shen, MS, ASCPCM, Manager of the Genomics Laboratory Service at Miami Cancer Institute, to discuss her team’s progress with virology research.
During early 2020, Weiming Shen was conducting cancer genomic profiling and biomarker research as usual. But when she started to notice the number of COVID-19 cases worldwide increasing drastically every day, she wondered if there were something she could do to contribute. Weiming and her colleagues believed they had both the tools and knowledge necessary for supporting this effort. They decided to rapidly shift the focus of their work.
Recently, Weiming and her team completed RNA extraction validation independently using RNAdvance Viral reagents from Beckman Coulter Life Sciences. RNA extraction is required to isolate COVID-19 viral RNA for downstream genomic applications. When asked why Weiming initiated COVID-19 research using RNAdvance Viral, she conceded the reagents were not, in fact, her first choice. Still, she was in urgent need of “an alternative RNA extraction solution due to an in-house kit shortage.”
Previous positive working experience with the Beckman Coulter Life Sciences Genomics team led her to reach out again to that team, which responded to her request immediately. To ensure a successful extraction evaluation, the Beckman team provided the reagents her team needed within 24 hours.
Weiming and her team first tested the RNA extraction efficiency of Beckman reagents using an RNA standard that was spiked into the viral transport media. The RNA extracted by RNAdvance Viral showed comparable Ct Values to their in-house kit, and is consistent with the RNA Standard control. RNAdvance Viral showed an analytical performance of about 1 copy/µL. Weiming’s team also observed a consistently lower Ct when compared to the in-house RNA extraction kit (Ct average: 35.1 vs. 36.7).
She and her team performed an RNA comparison study using her downstream RT-PCR process against their other qualified kit. They were pleased to discover that RNAdvance Viral showed showed 100% concordance for all samples.
Dianelis Mondejar Alvarez is setting up RT-qPCR using Biomek i5 (right).
Given the continuing global shortage of many RNA extraction reagents due to current demand, seeing this successful result makes Weiming feel exceptionally confident using the RNAdvance Viral as their alternative RNA extraction method for virology research. As she said, the most critical learning throughout this process is “It’s always good to be prepared with a backup plan.”
Virology research is vital in helping scientists fight the pandemic. With support from the Beckman team, Weiming and her team were able to complete the RNA extraction evaluation in less than one week.
Weiming was glad she reached out to the Beckman team because they are always committed, she says, “to (providing) fast technical support and reagents so quickly. Mostly, the willingness to help (the) customer in any way (they) can.”
Beckman Coulter Life Sciences is proud to be a small part of our customer’s fight against COVID-19. We appreciate that Weiming and her team took on the challenge to help allay some of the concern so many Americans have about this evolving pandemic.
She and her team are truly the real heroes of this story.
Melanhy Da Silva and internal team members are getting ready to transport samples between Baptist Hospitals and Miami Cancer Institute for RNA extraction.
Product Information: Viral RNA Extraction Reagent Kits
RNAdvance Viral Reagent Kit - 768 Preps
RNAdvance Viral XP Reagent Kit - 1056 Preps
RNAdvance Viral XP is listed as an extraction method for use with the Centers for Disease Control’s EUA-authorized COVID-19 test referenced in FDA’s FAQ on testing for SARS-CoV-2. Other than this designation, RNAdvance Viral XP is for research use, and not intended for diagnostic purposes.
RNAdvance Viral is for research use.
Watch the collaboration between Beckman Coulter Life Sciences and Integrated DNA Technologies (IDT) for the solutions to qPCR sample prep, featuring viral RNA extraction reagent kits.