Estrazione da Tessuti Fissati in Formalina e Inclusi in Paraffina (FFPE) (RNA)

Il sistema Agencourt FormaPure offre un processo semplice e compatibile con l'automazione per l'estrazione di acido nucleico totale da campioni di tessuto fissato in formalina e incluso in paraffina (Formalin-Fixed, Paraffin-Embedded, FFPE). Grazie all'utilizzo della tecnologia di immobilizzazione reversibile di fase solida (Solid Phase Reversible Immobilization, SPRI) basata su biglie paramagnetiche, il sistema Agencourt FormaPure non richiede filtrazione a vuoto, centrifugazione o solventi organici come fenolo o xilene. Il passaggio dalla digestione in paraffina all'estrazione di acido nucleico totale avviene in circa quattro ore (senza impostazione) per 96 campioni in formato multi-pozzetto mediante l'utilizzo della workstation Beckman Coulter Biomek NXP o FXP Span-8. I recuperi risultano significativamente più elevati rispetto a quelli offerti dalle tecniche concorrenti.

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FormaPure XL RNA Visual Workflow

Genomics FormaPure XL RNA Workflow

Product Specifications: FormaPure XL RNA

Application Uses RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic acid sample prep
Format Liquid
Starting Sample Material FFPE (formalin fixed paraffin embedded) tissue.
Automated Available Yes
Item Specifications Referenced C36000

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Title Description
Agencourt FormaPure Reagent Kits
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Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE
Total Nucleic Acid Isolation from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues using FormaPure XL Total on a KingFisher™ Duo Prime
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This protocol demonstrates the FormaPure XL Total performance on the KingFisher Duo Prime purification system. Automating chemistry can reduce the risk of human error, hands-on time (HOT) and total turn-around time (TAT).
Simultaneously DNA and RNA extraction from formalin-fixed paraffin embedded (FFPE) tissue
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In this poster we present a streamlined NGS ready to use sample prep workflow for FFPE tissue using a product in current development, FormaPure XL Total* (Beckman Coulter Life Science). FormaPure XL Total uses SPRI (Solid Phase Reverse Immobilization) paramagnetic beads to isolate DNA and RNA simultaneously.
AMP 2019: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
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In this poster, presented at AMP 2019, we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
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In this poster we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
FormaPure XL Data Sheet
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FormaPure XL reagent kits represent a single chemistry system designed and optimized for use in next-generation sequencing (NGS) as well as other downstream applications including qPCR/ddPCR/PCR
Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE Tissues
{279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}
FormaPure XL reagent kits represent a single-chemistry system with demonstrated capability for use in Next-Generation Sequencing (NGS) as well as other downstream applications including qPCR. Maximizing integrity, yield and purity from an FFPE sample is required to minimize the risk of losing important genetic information
Automated TruSeq RNA Sample Preparation from FFPE tissue specimens utilizing the Biomek FXp Liquid Handler
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This application note describes automated RNA library construction using the Beckman Coulter Biomek FXP Liquid Handler from archival FFPE tissue specimens.
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisher™ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.
FormaPure Total Quick Guide
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Checklist instructions for using the FormaPure Total extraction and purification kit.
FormaPure RNA: Extended Protocol for RNA Isolation from FFPE Sample
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This document provides instructions for using the FormaPure RNA extraction and purification kit.
FormaPure Total: Protocol for DNA and RNA Isolation from the Same FFPE Sample
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This document outlines the instructions for using the FormaPure Total extraction and purification kit.
FormaPureRNAクイックマニュアル
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
FormaPureRNAの日本語簡易版マニュアルです。使用前には正式なマニュアル（英文）を必ずご一読ください。
FormaPureXL DNAクイックマニュアル
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
FormaPureXL DNAの日本語簡易版マニュアルです。使用前には正式なマニュアル（英文）を必ずご一読ください。
SPRI Methodology
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Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.


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