Cell Sorter CytoFLEX SRT

CytoFLEX SRT Cell Sorter è un sorter da banco in grado di soddisfare un'ampia gamma di esigenze di sorting. Come la Piattaforma CytoFLEX, include tecnologie innovative che semplificano la configurazione e il funzionamento, consentendo ai ricercatori di concentrarsi sul lavoro di ricerca. Quando completamente attivata, la serieViolet-Blue-Yellow Green-Red (V-B-Y-R) dispone di 15 detectors. La versione base dispone di 5 laser; possono essere aggiunti su richiesta ulteriori laser e detector. È stato progettato per sorting complessi permettendo sorting con impostazioni differenti su ognuno dei 4 canali, compresa la possibilità di rilevare gli errori di sorting su uno degli altri canali. È possibile aggiungere il Biosafety Cabinet che  include la possibilità di espulsione degli aerosol. In alternativa, è disponibile una Aerosol Evacuation Unit indipendente.

  • Adatto a schemi di sorting complessi, con sorting a 4 canali, Mixed Mode sorting, e la possibilità di rilevare errori evidando di perdere cellule del campione
  • Setup semplificato, utilizza sistemi automatici per stabilire e mantenere il flusso sorting
  • Supporto da remoto tramite BeckmanConnect per il contatto coi nostri tecnici, e per praticare riparazioni di routine in maniera indipendente
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CytoFLEX nano Flow Cytometer Features

Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.

Sensitivity

With an unparalleled size sensitivity from 1µm to 40nm* and 10nm resolution,** the CytoFLEX nano flow cytometer enables the detection of smaller EV populations and their low-abundance cargo.

Flexibility

Open up your research with 6 fluorescent channels and 5 side scatter channels. Gather all the necessary data with fewer constraints.

Maximum Consistency

Maintaining contamination-free, robust instrument performance is critical when working with small size particles. Obtain reproducible results you can trust with >90% volumetric counting accuracy and <1% carryover between samples thanks to automated QC controls.

Familiarity

Built on the same principles as the CytoFLEX platform, the CytoFLEX nano flow cytometer features a similar footprint and user-friendly software interface.

 



The CytoFLEX nano analyzer utilizes  CytExpert for CytoFLEX Acquisition and Analysis Software and has the ability to count, size, and characterize EVs using a single technique, propelling your research forward.

Size  Count  Characterize
Particle size determination by flow cytometry requires calibration using an appropriately characterized reference particle paired with a calibration method. Latex polystyrene particles are the tool of choice for this task due to their NIST-traceable nature and reliably consistent manufacturing. Through the calibration method taught to each CytoFLEX nano flow cytometer customer, data will be reliably reportable in absolute units for method comparison and correlation. The most robust counting methods rely on highly controlled volumetric delivery of samples rather than hand loading samples with the implied variability. The CytoFLEX nano flow cytometer allows users to customize their sample handling so that the right amount of sample is used for the results needed. The ability to describe nanoscale particles requires best-in-class sensitivity. To make this possible, the CytoFLEX nano flow cytometer has completely redesigned the optics and fluidics system to maximize the detection of even the smallest amounts of fluorescence across 6 detection channels and excited by 4 collinear lasers.

Performance Characteristics

CytoFLEX nano Flow Cytometer Performance
 Violet side scatter sensitivity VSSC1: 40 nm relative to polystyrene nanoparticles
VSSC2: 80 nm relative to polystyrene nanoparticles
Scatter detection dynamic range VSSC 1 for small range 40-150 nm polystyrene nanoparticles
VSSC2 for large range 80-1000 nm polystyrene nanoparticles
Violet forward scatter sensitivity 300 nm relative to polystyrene nanoparticles
Fluorescence sensitivity and resolution

 Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity Fluorospheres

V477: 8 peaks

B531: 8 peaks

Y595: 8 peaks

R670: 6 peaks

R710: 5 peaks

R792: 4 peaks
 Fluorescence rCV CV < 10% (using QC Fluorospheres at 1 µL/min)
Carryover between Samples ≤1%a
 Acquisition speed Maximum electronic acquisition speed 16000 events/second with ≤ 95% yield
Recommended maximum sample acquisition speed 5000 events/second to avoid possible swarming or coincidence situations.
 Volumetric Counting accuracy > 90%b

Learn more about nanoscale flow cytometry

A comprehensive guide to extracellular vesicle counting, size determination, and characterization

CytoFLEX nano: Calibrating Light Scatter Data to Diameter using FCMPASS™

A new approach to nanoscale flow cytometry

The New Frontier of Nanoscale Flow Cytometry

Into The World Of Extracellular Vesicles

History of Extracellular Vesicles

Deciphering the Heterogeneity of Extracellular Vesicles

Expanding the Dimensions of Nanoscale Analysis by Flow Cytometry

Alfonso Blanco Testimonial

Advancing EV research - First Experiences with the CytoFLEX nano Flow Cytometer

Nanoscale Flow Cytometry

a. Tested with polystyrene nanoparticles

b. Tested with 144 nm QC Scatterspheres on 3 units under the conditions: record 3 minutes at the sample flow rates of 1µL/min, 2µL/min, 3µL/min, 4µL/min, 5µL/min, 6µL/min respectively, repeat 5 times, then calculate the average total events for each speed, then compare the calculated volumetric counting accuracy with the theoretical total events ( the known concentration 1.3*10 8 x volume).

*polystyrene when triggering on violet side scatter
** In development. Performance characteristics have not been validated.

For Research Use Only. Not for use in diagnostic procedures.