Quantitative Determination of Reaction Stoichiometry, Interaction Energies, and Solute Masses Using Analytical Ultracentrifugation
A brief but informative visual demonstration of the process of analytical ultracentrifugation, which enables live monitoring of macromolecules in solution under native, biologically relevant conditions
The analytical ultracentrifugation (AUC) technique is presented as a highly effective method for characterizing the structural aspects of macromolecules and as a quantitative technique that provides access to detailed thermodynamic characterization of a system under study. Also learn how AUC enables study of conformational changes and complex formation in physiologically relevant buffers. Dr. Andy Herr provides a great overview of analytical ultracentrifugation (AUC). He discusses basic theory of AUC, sedimentation velocity, sedimentation equilibrium, and more.