Pulizia del cDNA e dell'RNA
Il Agencourt RNAclean XP - Kit offre attraverso l'uso della tecnologia di immomibilizzazione in fase solida reversibile (SPRI) - tecnologia basata su biglie magnetiche, la purificazione semplice, flessibile ed eccezionalmente riproducibile di reazioni enzimatiche quali la sintesi di cDNA e in vitro trascrizione (IVT).
Choose a RNA and cDNA Model
RNAClean XP Workflow Steps
RNAClean XP purifies cDNA and RNA from common enzymatic reactions.
Efficient recovery of your sample ensures that critical data is not lost within the workflow.
Manual, hands-on cleanups using RNAClean XP require less than 30 minutes.
For higher throughput needs, RNAClean XP is automated on Biomek platforms enabling hundreds of samples per day.
|Application Uses||Purification & Clean-up, RNA Clean-up, cDNA Clean-up, IVT cleanup|
|Starting Sample Material||RNA or cDNA|
|Item Specifications Referenced||A63987|
Donega, V., Burm, S. M., van Strien, M. E., van Bodegraven, E. J., Paliukhovich, I., Geut, H., . . . Hol, E. M. (2019). Transcriptome and proteome profiling of neural stem cells from the human subventricular zone in Parkinson’s disease. Acta Neuropathologica Communications, 7(4). doi:10.1186/s40478-019-0736-0
- Used to clean up amplified RNA
Warner, A. D., Gevirtzman, L., Hillier, L. W., Ewing, B., & Waterston, R. H. (2019, May 23). The C. elegans embryonic transcriptome with tissue, time, and alternative splicing resolution. Genome Research. doi:10.1101/gr.243394.118
- Used to clean up RNA post rRNA removal
Caro-Vegas, C., Bailey, A., Bigi, R., Bamania, B., & Dittmer, D. P. (2019, January/February). Targeting mTOR with MLN0128 Overcomes Rapamycin and Chemoresistant Primary Effusion Lymphoma. mBio, 10(1), e02871-18. doi:10.1128/mBio.02871-18
- Used to clean up fragmented RNA and cDNA
Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.