Isolamento da Sangue (DNA)

Agencourt Genfind v2

Il kit Agencourt Genfind v2 viene utilizzato per estrarre DNA genomico (gDNA) da sangue intero, plasma, siero, prelievi di mucosa della bocca, saliva e FTA Cards. Grazie alla tecnologia SPRI ((Solid Phase Reversible Immobilization), basata sull'utilizzo di biglie magnetiche, è possibile ottenere un rendimento elevato e riproducibile di gDNA per diversi tipi di applicazioni. La tecnologia SPRI permette una purificazione più facile e un'automazione più semplice dei flussi campione (utilizzando un pipettatore Biomek) rispetto ai metodi tradizionali basati sulla centrifugazione o la filtrazione sotto vuoto.

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Sistemi di manipolazione dei liquidi

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GenFind V3 Isolation Workflow

Genomics GenFind V3 Workflow

Caratteristiche

Overview
GenFind V3 isolates genomic DNA (gDNA) from whole blood and serum.

No Inhibition from Anticoagulants
GenFind V3 efficiently removes common anticoagulant and contaminants from blood such as citrate, EDTA, and heparin.

Product Specifications

Application Uses DNA Isolation, DNA Extraction, DNA sequencing, Genomic DNA
Format Liquid
Starting Sample Material Blood
Automated Available Yes
Item Specifications Referenced A83078

Documentation and Related Materials

Title Description
GenFind V3 DataSheet
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The method can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling workstations
DNA extraction from up to 2 mL blood using GenFind V3
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Here we present a highquality, research-ready DNA extraction method from large volume whole blood by using GenFind V3.
DNA extraction from Zeesan@ Saliva DNA sample collection kit using GenFind V3
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Saliva contains buccal epithelial cells and white blood cells which provide rich genetic data. Due to the easy access and non-invasive character, saliva is an alternative to blood collection. Here we present a high-quality, DNA extraction method from saliva using GenFind V3.
DNA Extraction from Filamentous Fungi using GenFind V3
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This protocol provides two other methods for lysis that are more automation friendly and we show that they produce results that are very similar to the industry standard of bead beating.
Viral Nucleic Acid extraction using RNAdvance Blood
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The method presented here is a modified RNAdvance Blood protocol that can extract both RNA and DNA from viral samples.
DNA Isolation from Bacteria using GenFind V3
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This protocol provides a method for extracting DNA from bacteria with data showing its effectiveness with Escherichia coli, Staphylococcus aureus, Salmonella, Corynebacterium and Mycobacterium, a difficult to work with bacteria
DNA extraction FTA cards or filter paper blood spots using GenFind V3
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This method provides instruction for additional lysis buffer volumes and optimized lysis conditions for extraction of DNA
Whole Blood DNA Extraction from PAXgene Blood DNA Tube using GenFind V3
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Here we show that the GenFind V3 protocol can process whole blood directly from PAXgene™ Blood DNA Tubes with no centrifugation step necessary
DNA extraction from Mouthwash samples using GenFind V3
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Mouthwash samples are taken in large volumes (usually 10 - 30 mL), and cells need to be spun down from the total volume of mouthwash before extraction can begin. This protocol incorporates those steps and optimizes the lysis for mouth cell samples
Extraction of DNA from Mucoid Bacteria using GenFind V3
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This protocol reduces viscosity due to the presence of exopolysaccharides and decreases the amount of polysaccharide in the final eluate.
DNA extraction from fresh/frozen tissue using GenFind V3
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This provides a solution that allows a user to use one kit to extract DNA from multiple sample types with small modifications in the workflow.
DNA extraction from cell culture using GenFind V3
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This protocol provides a method for extracting DNA from cultured cells (≤ 2 million cells). Data shown shows the efficiency of this procedure with 1.4 million Jurkart cells, an immortalized line of human T lymphocyte cells
Comparison between Mutation Profiles of Paired Whole Blood and cfDNA Samples
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Review our poster we presented at ASHG 2019 where we looked at the data associated with the comparison between mutation profiles of paired whole blood and cfDNA samples
Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation
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Here, we demonstrate a walk-away automated solution for GenFind V3 kit to purify up to 400 µL of whole blood using the Beckman Coulter Biomek i7 Hybrid Genomics Workstation.
GenFind V3: Automated genomic DNA extraction from large volume whole blood
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In this poster we present an automated, high-quality, research-ready DNA extraction method from large volume blood. GenFind V3 DNA isolation kit automated on the KingFisher Duo Prime system provides scalable and consistent system for gDNA extraction from 2 mL of whole blood.
Viral Nucleic Acid Extraction
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In this application note, we report the ability to extract DNA from samples containing HBV. The method presented here is a modified protocol using RNAdvance Blood that can extract nucleic acids from DNA and RNA viruses.


Technical Documents