Rimozione Sanger Sequenziamento Dye Terminator

Il kit Agencourt CleanSEQ è basato sulla tecnologia SPRI a biglie magnetiche, per purificare i prodotti di sequenziamento dopo una reazione di amplificazione. Il protocollo prevede solo 3 passaggi, non necessita di centrifugazione né di filtrazione. Il sistema Agencourt CleanSEQ fornisce all'utente un metodo di purificazione adattabile alle esigenze del laboratorio, semplice ed economico e facilmente automatizzabile con i pipettatori della serie Biomek.

Choose a Size Selection Model

SPRIselect Left Workflow

Genomics SPRIselect Left Side Size Selection Workflow

SPRIselect Right Workflow

Genomics SPRIselect Right Side Size Selection Workflow

Product Features

Overview
SPRIselect chemistry speeds and simplifies nucleic acid size selection for fragment library preparation for next-generation sequencing.

Customizable Size Selection
SPRIselect allows tunable size selection from 150 to 800 base pairs to adjust to specific workflow needs.

Consistent Results
SPRIselect yields consistent size selections without bead calibration between reagent lots.

Flexible and Scalable
Suitable for manual or automated workflows - the SPRIselect method can be run on a variety of Biomek platforms that offer minimal hands-on time.

Product Specifications

Application Uses Purification & Clean-up, Nucleic acid sample prep, DNA Size Selection, RNA Size Selection, PCR Clean-up, NGS Clean-up, PCR clean-up
Format Liquid
Starting Sample Material Nucleic Acid
Automated Available Yes
Item Specifications Referenced B23318

Citations

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-5

  • Size Selection of Libraries

Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz181

  • Used on cDNA Libraries

Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.057

  • Used on ChIP-seq Libraries

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Documentation and Related Materials



Technical Documents