Vi-CELL XR

Determinazione automatica del numero, della concentrazione e della vitalità delle cellule mediante l'analisi dell'immagine

Vi-CELLTM XR combina il metodo analitico e l'utilizzo del blu tripano per eseguire il conteggio automatico delle cellule tramite l'analisi delle immagini digitali. Lo strumento esegue in modo standardizzato e automatico le diverse fasi dell'analisi, dal prelievo di alcuni µl di sospensione cellulare alla colorazione con blu tripano fino all'analisi e la pulizia della cellula Il campionatore a 12 posti può essere ricaricato di continuo. La preparazione dei campioni è pertanto automatizzata.

Vi-CELLTM XR permette al responsabile del controllo di processo di monitorare costantemente i parametri come il tasso di crescita o tempo di raddoppio. In alternativa è possibile integrare Vi-CELLTM XR con "stazioni di lavoro cellulare" più grandi e con altre soluzioni automatizzate. Vi-CELLTM XR è conforme a 21 CFR Parte 11.

Vi-Cell XR

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Caratteristiche

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Tech Docs Part Number Product Name Description Particle Size Analysis Range Viability Range List Price Buy
383556
Vi-CELL XR Cell Viability Analyzer System
2.00|70.00|μm / 2|70|μm

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731196
VI-CELL XR System without Computer
Vi-CELL XR System, Quad Paks, Sample Cups, Control
2.00|70.00|μm / 2|70|μm
0.00|100.00

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731050
Vi-CELL™ XR Complete System
Vi-CELL XR System, Quad Paks, Sample Cups, Control, Computer, Monitor
2.00|70.00|μm / 2|70|μm
0.00|100.00

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383721
Vi-CELL™ XR 4 Bags of Sample Vials, 120 per bag
4 bags of 120 sample vials per bag

ERROR: Field "Particle Size Analysis Range" does not exist on this item

ERROR: Field "Viability Range" does not exist on this item

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383722
VI-CELL™ XR Quad Pack Reagent Kit with 8 Bags of Sample Vials, 120 per bag
4 reagent kits, 8 bags of 120 sample vials

ERROR: Field "Particle Size Analysis Range" does not exist on this item

ERROR: Field "Viability Range" does not exist on this item

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383260
Vi-CELL™ XR Single Reagent Pack with 120 Sample Vials
1 reagent kit, 1 bag of 120 sample vials

ERROR: Field "Particle Size Analysis Range" does not exist on this item

ERROR: Field "Viability Range" does not exist on this item

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175474
Vi-CELL™ Focus Control
Focus Control

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175478
Vi-CELL™ XR 1M Concentration Control
1M Concentration Control

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B94987
Vi-CELL™ XR Quad Pack Reagent Kits for Integrated Systems
4 reagent kits, no sample vials included

ERROR: Field "Particle Size Analysis Range" does not exist on this item

ERROR: Field "Viability Range" does not exist on this item

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Caratteristiche

Caratteristiche dell'apparecchio

  • Intervallo di misura: da 2 a 70 μm (diametro)
  • Tipo di videocamera/risoluzione: CCD/0,68 µm/pixel
  • Numero massimo di foto: 100
  • Tempi di misura standard: 150 secondi
  • Volume dei campioni: 500 µl (per 5 x 104 – 1 x 107 cellule/ml)

Metodo automatico al blu tripano

  • Tempi di analisi ridotti e nessuna analisi manuale al microscopio.
  • Una telecamera CCD (con risoluzione di 0,68 µm/pixel) permette di analizzare più di 15.000 cellule.
  • Analisi della vitalità per mezzo di "modelli cellulari" specifici.
  • Campionatore automatico ricaricabile durante la misura, per il trattamento automatico dei campioni

Software

  • Visualizzazione della ripartizione per numero, concentrazioni e dimensioni
  • Determinazione della vitalità cellulare (in valore assoluto, % e cellule/ml)
  • Calcolo del numero e della distribuzione degli aggregati
  • Visualizzazione e calcolo della circolarità
  • Determinazione del tasso di crescita e del tempo di raddoppio per i bioprocessi

Validazione e certificazione

  • Conforme a 21 CFR Part 11
  • Registrazione delle operazioni e protezione con password
  • Soglie di sicurezza e firma elettronica
  • Programma V-Check per SQ, DQ, IQ, OQ


Tabella delle specifiche

Detection Video image through quartz cell
Throughput < 2.5 min
Max Capacity Volume Range 0.50|2.00|mL
Sample Volume 500.00|µL

Documentazione

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Title Description
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Authors: S. E. SZABO, S. L. MONROE, S. FIORINO, J. BITZAN, K. LOPER | The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, Maryland; Beckman Coulter Miami, Florida, USA | Published 2004
Use of Beckman Coulter Reagents for Automated, Whole Blood, White Blood Cell Viability Measurements
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Authors: Stephen E. Szabo, Ph.D. | Sarah Monroe, B.S.| Beckman Coulter, Inc | Published 2004
Instrument-to-Instrument Variability in the Vi-CELL Automated Viability Analyzer
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Authors: Clarence Lew, Senior Applications Scientist, John A. Gomez, Senior Staff Technical Operations Engineer, Matthew N. Rhyner, Ph.D., Global Marketing Manager-Analytical Products | Beckman Coulter Life Sciences, Brea, CA USA | Published 2012
Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi-Cell™ XR
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Authors: Stephen E. Szabo, Ph.D. | Sarah Monroe, B.S. | Beckman Coulter, Inc. | Published 2004
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters.
Quantification of Cellular Toxicity with Particle Characterization and Centrifugation
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Beckman Coulter tools enable quantification of cellular toxicity caused by nanoparticles, reducing time to remove aggregated particles and quantifying cell toxicity in the presence of single-walled carbon nanotubes.
Vi-CELL XR Cell Viability Analysis Brochure
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Yeast Cell Analyses Using the Beckman Coulter® Vi-CELL™
{A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}
The Vi-CELL XR provides the high resolution necessary for accurate results on yeast and other small cells
Easy ... Fast ... Reliable Vi-CELL XR Cell Viability Analyzer Product Brochure
{A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}
The Vi-CELL software interface has been designed for simplicity; yet offers numerous innovative features for those users demanding maximum flexibility
Insect Cell Analyses Using the Beckman Coulter® Vi-CELL™
{A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}
Insect cells are used to produce therapeutic proteins
Jurkat Cell Analyses Using the Beckman Coulter® Vi-CELL™
{A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}
Jurkat cells are human leukemic T lymphocytes
Manufacturing Processes for Engineered T-Cell Therapy – CAR-T
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}|{279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}|{749D68FB-4893-4B2D-BD6C-C662F0520F64}
Lentiviral Vector Production Technical Application Note
{611E05FD-FC41-4E23-9498-DA5662F8D4F9}
Clone gene of interest into a modified Lentiviral vector with this production method protocol
Vi-CELL® XR Cell Type Tutorial
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
Introduce users to the Cell Type Feature in the Vi-CELL XR software
The Particle Analyzers Advancing Life Science Research
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Scientists share how automated cell counting is revolutionizing research

Technical Documents