Estrazione da Cellule
Il kit Agencourt RNAdvance Cell v2 si basa sulla tecnologia di immobilizzazione reversibile di fase solida (Solid Phase Reversible Immobilization, SPRI), che utilizza biglie paramagnetiche, nota per la sua capacità di sintetizzare costantemente acidi nucleici puri dal livello ottimale di qualità e rendimento. Il sistema offre anche un elevato livello di recupero e di purezza dell'RNA senza la necessità di filtrazione o di centrifugazione. Le proprietà chimiche del kit Agencourt RNAdvance Cell v2 si basano su un processo compatibile con l'automazione per l'isolamento di RNA totale per applicazioni di microarray e qPCR in tempo reale.
Choose a From Cells Model
RNAdvance Cells Workflow Steps
Product Features
RNAdvance Cell V2 isolates total RNA from cultured cells.
The RNAdvance Cell V2 isolation kit purifies RNA with high RIN scores enhancing suitability for downstream applications.
Combining RNAdvance Blood chemistry with our Biomek Workstation offers high throughput and minimal hands-on solutions: two 96-well plates of samples can be purified in approximately 2 hours and 15 minutes.
Product Specifications
| Application Uses | RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic acid sample prep |
| Format | Liquid |
| Starting Sample Material | Cultured Cells |
| Automated Available | Yes |
| Item Specifications Referenced | A47943 |
Citations
Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113
- Used to extract RNA from ESC and EpiSC cell lines
Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025
- Used to extract RNA from MCF-7 cells
Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.