Ultracentrifugazione analitica

A differenza di altri metodi usati per analizzare macromolecole, l'ultracentrifugazione analitica (analytical ultracentrifugation, AUC) consente la caratterizzazione di campioni nel loro stato nativo in condizioni di soluzione biologicamente rilevanti.L'AUC è la tecnologia più versatile, rigorosa e accurata disponibile per determinare il peso molecolare, le proprietà idrodinamiche e termodinamiche di una proteina o altre macromolecole. Può essere usata per indagare su quasi qualsiasi tipo di molecola o particella in un'ampia gamma di concentrazioni e in una assortita varietà di solventi. Per molte domande di ricerca non esiste un sostituto analitico soddisfacente della AUC.

A new dimension in simplified AUC analysis - Dr. Borries Demeler

Watch this testimonial on the Optima AUC, analytical ultracentrifuge from Dr. Borries Demeler, Professor of Biochemistry and Structural Biology. Dr. Demeler is the creator of Ultrascan, an AUC software analysis program. Hear about his experience with the newest analytical ultracentrifuge.

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Title	Description
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	AUC provides a matrix-free environment that allows the near-native state characterization of a wide range of molecules. Our recently launched Optima AUC is the only commercially available instrument equipped with a state of the art optical system.
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Proteins have a variety of cellular functions, structures, and mechanisms of action. Routinely, proteins bind other biomolecules, or ligands, in order to complete a task. Researchers gain valuable knowledge on how proteins work in a cellular environment by purification of proteins bound to their appropriate substrate.
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Insulin is a well characterized drug used to treat diabetes. A large number of insulin formulations have been investigated in order to develop the optimum time delivery suited to patient needs. In addition, a number of insulin analogs such as Insulin Glargine and LysPro insulin (Humalog) have been developed and marketed as derivatives for immediate use at meal time and a more sustained release.
Rapid Determination of Spectrophotometric Absorptivity by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: R. A. Gray, A. Stern, T. Bewley, and S. J. Shire Genentech, Inc. \| South San Francisco, CA
Linearity of BSA Using Absorbance & Interference Optics {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dean Clodfelter, M.S., \|Chad Schwartz, Ph.D., Beckman Coulter Inc., 5350 Lakeview Parkway Dr., Indianapolis, IN 46268
Stability of the Human Immunodeficiency Virus-1 Reverse Transcriptase Heterodimer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jacob Lebowitz - University of Alabama, Birmingham, AL
High-Performance Automation and Centrifugation Preparation of Carbon Nanotubes for Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Single-Walled Carbon Nanotubes (SWCNT) experienced increasing interest in the past 15 years for semiconductors, fuel cells, and biomedical applications
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph.D., Dean Clodfelter, M.S. \| Beckman Coulter, Inc., Indianapolis, IN 46268
The Solution Conformation of Novel Antibody Fragments Studied Using the Proteomelab XL –A Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Peter J. Morgan, Olwyn D. Byron, Stephen E. Harding \| Department of Applied Biochemistry and Food Science \| University of Nottingham, Sutton Bonington, U. K.
Analysis of Membrane Protein Dimerization by Sedimentation Equilibrium {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Karen G. Fleming - Department of Molecular Biophysics and Biochemistry, New Haven, CT
Chymotrypsin: Characterization of a Self-Associating System in the Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Paul Voelker and Don McRorie \| Beckman Coulter
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Julia P Luciano-Chadee - Beckman Coulter, Inc., Indianapolis, IN \| Chad Schwartz Ph.D. - Beckman Coulter, Inc., Indianapolis, IN
Short Column Sedimentation Equilibrium Analysis for Rapid Characterization of Macromolecules in Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Thomas M. Laue University of New Hampshire Department of Biochemistry Durham, NH 03824
An Analytical Revolution: Introducing the Next Generation Optima AUC {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Author: Chad Schwartz, Ph.D. \| Beckman Coulter Life Sciences
Enrichment and Characterization of Ferritin for Nanomaterial Applications {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Rodolfo GhirlandoƗ, Radina Mutskova¥, Chad SchwartzɈ \| National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health Honors College, Integrated Life Sciences, University of Maryland \|Beckman Coulter, Inc., Indianapolis, IN, 46268
Enrichment and Characterization of Ferritin for Nanomaterial Applications {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Rodolfo Ghirlando \| Radina Mutskova \| Chad Schwartz
ProteomeLabtm XL-A/XL-I Protein Characterization System {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Native condition protein characterization with Beckman Coulter.
Determination of Molecular Weight of Glycoproteins by Analytical Ultracentrifugation {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	Beckman Coulter Life Sciences
A Guide to Winning a Shared Instrumentation Grant (SIG) {749D68FB-4893-4B2D-BD6C-C662F0520F64}	The Shared Instrumentation Grant (SIG) Program provides funds for purchasing shared-use instrumentation not typically supported by other funding programs sponsored by the National Institutes of Health (NIH).
Self-Associating Systems in the Analytical Ultracentrifuge {3AB28F00-B7FC-47D9-80F2-C0E49A388F7B}	Authors: DONALD K. MCRORIE and PAUL J. VOELKER \| Beckman Coulter
Applications Enabled By Multi-Wavelength Analysis With The Optima AUC {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Instruments and components ordering information.
An Overview Of Analytical Ultracentrifugation (AUC) {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Beckman Coulter Instruments
g-Max: Added Capabilities to Beckman Coulter's versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The g-Max System adds several valuable capabilities to your Beckman Coulter ultracentrifuge rotors:
An Analytical Revolution: Introducing the Next Generation Optima AUC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Optima AUC is the latest innovation from Beckman Coulter and offers significant advantages as an analytical ultracentrifugation platform over the predecessor ProteomeLab.
Analytical Ultracentrifugation Training {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Real-world training to help maximize the value of your AUC
Introduction to Analytical Ultracentifugation {3AB28F00-B7FC-47D9-80F2-C0E49A388F7B}	Author: GREG RALSTON - DEPARTMENT OF BIOCHEMISTRY \| THE UNIVERSITY OF SYDNEY - SYDNEY, AUSTRALIA
Optima AUC - Absorbance, Interference
Optima AUC Overview, New Analytical Ultracentrifuge {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief overview of some of the features of the Optima AUC analytical ultracentrifuge that make it the most robust research technology available for thorough analysis of proteins, viruses and other macromolecules in their native, matrix-free state
AUC Insights - March 2019 {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}
A new dimension in simplified AUC analysis - Dr. Borries Demeler {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	Watch this testimonial on the Optima AUC, analytical ultracentrifuge from Dr. Borries Demeler, Professor of Biochemistry and Structural Biology. Dr. Demeler is the creator of Ultrascan, an AUC software analysis program. Hear about his experience with the newest analytical ultracentrifuge.
Velocity Method Example
An Analytical Revolution: Introducing the Next Generation Optima AUC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Analytical Ultracentrifugation - Macromolecule Characterization in Solution (Academia) {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Beckman Coulter delivered the first AUC sample characterization tool to the scientific community powering discoveries
Analytical Ultracentrifugation - Macromolecule Characterization in Solution (Biopharma) {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Beckman Coulter delivered the first AUC sample characterization tool to the scientific community powering discoveries
Applications of Counterflow Centrifugal Elutriation CCE	Applications of Counterflow Centrifugal Elutriation CCE and the history and principle behind it
Analytical Ultracentrifugation of Carbon Nanotubes	Sedimentation velocity (SV) experiments on dispersed nanoparticles present an effective means for determining the hard-to-evaluate properties of nanoparticles. This webinar by Dr. Jeffrey Fagan focuses on leveraging the capabilities of SV methods for characterizing single-wall carbon nanotubes (SWCNTs). Results detailing the use of density contrast techniques on the characterization of the interfacial layer on specific SWCNT species demonstrate the power of analytical ultracentrifugation to resolve this interfacial layer. Dr. Jeffrey Fagan is from the National Institute of Standards and Technology and discusses the analytical ultracentrifugation of single-wall carbon nanotubes, as well as provides background information to apply to other nanoparticles. Dr. Fagan begins with an introduction to analytical ultracentrifugation and a quick explanation of single-wall carbon nanotubes. He discusses what we can characterize with the AUC technique, problems and pitfalls we might encounter, and a discussion about how those pitfalls can differ for different nanoparticles. Lastly, he provides two technical examples using the AUC measurement technique on single-wall carbon nanotubes.
Analytical Ultracentrifugation For Structural Analysis - Proteins and Macromolecular Complexes	The analytical ultracentrifugation (AUC) technique is presented as a highly effective method for characterizing the structural aspects of macromolecules and as a quantitative technique that provides access to detailed thermodynamic characterization of a system under study. Also learn how AUC enables study of conformational changes and complex formation in physiologically relevant buffers. Dr. Andy Herr provides a great overview of analytical ultracentrifugation (AUC). He discusses basic theory of AUC, sedimentation velocity, sedimentation equilibrium, and more.
Analytical Ultracentrifugation in Nanoparticle Analysis	Joshua Robinson covers the utility of fractionation in the investigation of complex mixtures and polydisperse samples as well as versatility of analytical ultracentrifugation (AUC) for colloid chemistry. He quickly reviews analytical ultracentrifugation (AUC) theory, then discusses deconstructing nano-constructs and how AUC has been used in recent literature to really probe the underlying structure of nano-constructs, nanomaterials. Other topics covered include nanoparticle sample preparation, as well as Beckman Coulter's research and future perspectives.
Exploring the Stoichiometry of Macromolecular Complexes Using Multi-Signal Sedimentation Velocity Analytical Ultracentrifugation	This webinar by Dr. Chad Brautigam covers the stoichiometry of macromolecule complexes by multi-signal sedimentation velocity analytical ultracentrifugation.
Introduction to AUC - an Analytical Ultracentrifuge {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief but informative visual demonstration of the process of analytical ultracentrifugation, which enables live monitoring of macromolecules in solution under native, biologically relevant conditions
The History of AUC - An Analytical Ultracentrifuge {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief review of highlights in the long history of analytical ultracentrifugation -- from the 1920s through the dawn of the 21st century -- focused on its importance in the fields of science, medicine and industry
Optima Analytical Ultracentrifuge Absorbance
Optima AUC - Absorbance/Interference Bundle with An-60 Ti Rotor
Quantifying Protein Aggregates by Sedimentation Velocity	Webinar covering protein aggregate quantification by sedimentation velocity by Michael Stoner PhD of Amgen - PART I. AUC instrumentation & protein aggregate quantification; PART II. Size exclusion chromatography vs sedimentation velocity; PART III. Simulation as a tool for understanding results.
Physical and Chemical Characterization of Nanoparticle Constructs Using the Analytical Ultracentrifuge	Webinar covers physical and chemical characterization of nanoparticle constructs using analytical ultracentrifugation. Webinar is presented by Osman M Bakr from King Abdullah University of Science and Technology.
Quantitative Determination of Reaction Stoichiometry, Interaction Energies, and Solute Masses Using Analytical Ultracentrifugation	Live broadcast covering applications of analytical ultracentrifugation (AUC).
Analytical Methods to Measure Empty and Full AAV Particles	Review around biophysical characterizing techniques and a focus on the use of analytical ultracentrifugation (AUC) for measuring empty and full AAV particles
Analysis of Particle Size Distributions by Analytical Ultracentrifugation	Helmut Cölfen discusses and particle size distributions by analytical ultracentrifugation.
Analytical Ultracentrifugation in the Biopharmaceutical Industry	Dr. Alexander Bepperling focuses on the data analysis tools to achieve high precision when analyzing biopharmaceutical products due to an interest in minor species of about 1-2% usually comprising of the total protein content. He focuses on the data analysis done in SEDFIT. Learn how to set up continuous c(S) distribution analysis as implemented with SEDFIT software for enhanced reproducibility during a study of many batches of one protein. Also covers applications of sedimentation equilibrium (SE) for screening weak interactions and determining detergent binding to proteins.
Reflections on 70 Years of Centrifugation Innovation {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	Beckman Coulter presents 70 years of innovation in centrifugation from the perspective of 4 customers from around the world. Hear from Dr. Matt Perugini in Melbourne, Australia, Dr. Yuji Kobayashi in Osaka, Japan, Pert Kalmikov in Moscow, Russia, and Dr. Ashutosh Kumar in Mumbai, India.
Looking Under the Hood: Getting Started with the Optima AUC	n the webinar we discussed the fundamentals of analytical ultracentrifugation and looked at a few sample applications. <h3></h3>
Webinar: An Intro to Characterization of Biomolecules Using AUC	<span style="background-color: #ffffff; color: #5b5b5b;">Analytical Ultracentrifugation (AUC) is one of the most powerful biophysical tools used today for the characterization of biological samples ranging from small drug molecules to intact viruses, vesicles and microparticles</span>
Principles of Continuous Flow Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Continuous flow centrifugation is a laboratory time-saver, whereby large volumes of material can be centrifuged at high centrifugal forces without the tedium of filling and decanting a lot of centrifuge tubes, or frequently starting and stopping the rotor.
Advances in Sedimentation Analysis by Dr. Borries Demeler	This presentation covers advances in sedimentation velocity (SV) analysis including methods for accurately characterizing heterogeneous systems at high resolution; approaches to extract rich information from SV experiments and techniques to measure the strength of macromolecular interactions with discrete binding models to whole boundary SV experiments in analytical ultracentrifugation.
The Use of the Analytical Ultracentrifuge to Characterize Very Large Complex Biopharmaceuticals	This webinar covers the key attributes of AUC as a highly effective technique for characterizing viral vectors for viral gene therapy delivery systems